y atom sh2 biosensor Search Results


human grb2 (sh2 domain) antibody  (Bio-Techne corporation)
90
Bio-Techne corporation human grb2 (sh2 domain) antibody
Human Grb2 (Sh2 Domain) Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human grb2 (sh2 domain) antibody/product/Bio-Techne corporation
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human grb2 (sh2 domain) antibody - by Bioz Stars, 2026-04
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y atom sh2 biosensor  (Addgene inc)
93
Addgene inc y atom sh2 biosensor
HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM <t>SH2</t> and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
Y Atom Sh2 Biosensor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/y atom sh2 biosensor/product/Addgene inc
Average 93 stars, based on 1 article reviews
y atom sh2 biosensor - by Bioz Stars, 2026-04
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y atom sh2 y atom wdr5  (Addgene inc)
93
Addgene inc y atom sh2 y atom wdr5
HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM <t>SH2</t> and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
Y Atom Sh2 Y Atom Wdr5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/y atom sh2 y atom wdr5/product/Addgene inc
Average 93 stars, based on 1 article reviews
y atom sh2 y atom wdr5 - by Bioz Stars, 2026-04
93/100 stars
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sh2 and sh3 domains  (Horita Co Inc)
90
Horita Co Inc sh2 and sh3 domains
HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM <t>SH2</t> and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
Sh2 And Sh3 Domains, supplied by Horita Co Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sh2 and sh3 domains/product/Horita Co Inc
Average 90 stars, based on 1 article reviews
sh2 and sh3 domains - by Bioz Stars, 2026-04
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cdnas for the plc-g ph(n)-sh2-sh2-sh3-ph(c) domain  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc cdnas for the plc-g ph(n)-sh2-sh2-sh3-ph(c) domain
HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM <t>SH2</t> and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
Cdnas For The Plc G Ph(N) Sh2 Sh2 Sh3 Ph(C) Domain, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdnas for the plc-g ph(n)-sh2-sh2-sh3-ph(c) domain/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
cdnas for the plc-g ph(n)-sh2-sh2-sh3-ph(c) domain - by Bioz Stars, 2026-04
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sh2 array  (Panomics Inc)
90
Panomics Inc sh2 array
HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM <t>SH2</t> and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
Sh2 Array, supplied by Panomics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sh2 array/product/Panomics Inc
Average 90 stars, based on 1 article reviews
sh2 array - by Bioz Stars, 2026-04
90/100 stars
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tandem sh2: 10 kda filters  (Vivascience Inc)
90
Vivascience Inc tandem sh2: 10 kda filters
HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM <t>SH2</t> and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
Tandem Sh2: 10 Kda Filters, supplied by Vivascience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tandem sh2: 10 kda filters/product/Vivascience Inc
Average 90 stars, based on 1 article reviews
tandem sh2: 10 kda filters - by Bioz Stars, 2026-04
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ext1-sh2  (Genechem)
90
Genechem ext1-sh2
HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM <t>SH2</t> and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
Ext1 Sh2, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ext1-sh2/product/Genechem
Average 90 stars, based on 1 article reviews
ext1-sh2 - by Bioz Stars, 2026-04
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plasmids expressing hist1h4h-sh1  (Genechem)
90
Genechem plasmids expressing hist1h4h-sh1
HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM <t>SH2</t> and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
Plasmids Expressing Hist1h4h Sh1, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmids expressing hist1h4h-sh1/product/Genechem
Average 90 stars, based on 1 article reviews
plasmids expressing hist1h4h-sh1 - by Bioz Stars, 2026-04
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genes sh1, sh2, sh4, sh5  (Abbott Laboratories)
90
Abbott Laboratories genes sh1, sh2, sh4, sh5
HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM <t>SH2</t> and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
Genes Sh1, Sh2, Sh4, Sh5, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genes sh1, sh2, sh4, sh5/product/Abbott Laboratories
Average 90 stars, based on 1 article reviews
genes sh1, sh2, sh4, sh5 - by Bioz Stars, 2026-04
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anti-sh2-b antibody sh2-b  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare anti-sh2-b antibody sh2-b
HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM <t>SH2</t> and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
Anti Sh2 B Antibody Sh2 B, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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grb14(sh2  (Qiagen)
90
Qiagen grb14(sh2
HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM <t>SH2</t> and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
Grb14(Sh2, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/grb14(sh2/product/Qiagen
Average 90 stars, based on 1 article reviews
grb14(sh2 - by Bioz Stars, 2026-04
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Image Search Results


HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM SH2 and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.

Journal: Bio-protocol

Article Title: Sensitive and Adaptable Turn-On Maturation (ATOM) Fluorescent Biosensors for Detecting Subcellular Localization of Protein Targets in Cells

doi: 10.21769/BioProtoc.5239

Figure Lengend Snippet: HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM SH2 and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.

Article Snippet: HeLa human epithelial cells (ATCC, catalog number: CCL-2) 4. pCMV-MBP-yATOM_SH2 for expressing y-ATOM SH2 biosensor in mammalian cells (Addgene plasmid # 209708; http://n2t.net/addgene:209708 ; RRID: Addgene_209708) 5. pCMV-MBP-yATOM_WDR5 for expressing y-ATOM WDR5 biosensor in mammalian cells (Addgene plasmid # 209706; http://n2t.net/addgene:209706 ; RRID: Addgene_209706) 6. pCMV-MBP-yATOM_mCherry for expressing y-ATOM mCh biosensor in mammalian cells (Addgene plasmid # 209707; http://n2t.net/addgene:209707 ; RRID: Addgene_209707) 7. pCMV-MBP-yATOM_RAS for expressing y-ATOM RAS biosensor in mammalian cells (Addgene plasmid # 209709; http://n2t.net/addgene:209709 ; RRID: Addgene_209709) 8. pCMV-RBP_WDR5 for expressing WDR5 in mammalian cells (Addgene plasmid # 209702; http://n2t.net/addgene:209702 ; RRID: Addgene_209702) 9. pCMV-mCherry (Y70A) for expressing the Y70A (nonfluorescent) mutant of mCherry in mammalian cells (Addgene plasmid # 209703; http://n2t.net/addgene:209703 ; RRID: Addgene_209703) 10. pCMV-SH2 for expressing SH2 domain in mammalian cells (Addgene plasmid # 209704; http://n2t.net/addgene:209704 ; RRID: Addgene_209704) 11.

Techniques: Transfection, Plasmid Preparation, Expressing, Standard Deviation

HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM SH2 and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.

Journal: Bio-protocol

Article Title: Sensitive and Adaptable Turn-On Maturation (ATOM) Fluorescent Biosensors for Detecting Subcellular Localization of Protein Targets in Cells

doi: 10.21769/BioProtoc.5239

Figure Lengend Snippet: HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM SH2 and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.

Article Snippet: Plasmids expressing other validated biosensors (y-ATOM SH2 , y-ATOM WDR5 , and y-ATOM RAS ) and their ligands are also available from Addgene (Biological materials 4–11).

Techniques: Transfection, Plasmid Preparation, Expressing, Standard Deviation